APCAD, a novel method for detection of the full spectrum of chromosome abnormalities in preimplantation embryos.

Chromosome abnormalities are very frequent in early human IVF embryos and are considered the main cause of implantation failure and miscarriage.

Chromosome abnormalities are very frequent in early human IVF embryos and are considered the main cause of implantation failure and miscarriage. Current detection methods such as low coverage massive parallel sequencing (MPS) are fast and simple, but are unable to capture the full spectrum of chromosome abnormalities. Other methods, based on haplotyping (tracing chromosomes from generation to generation) can be used to detect these anomalies but large scale application is difficult as these methods require DNA from relatives of the prospective parents. 

We provide a novel and innovative method called 'Analysis of Parental contribution for aneuploidy detection' or 'APCAD' to detect and quantify all types of chromosome abnormalities by calculating the proportion of maternal (and hence paternal) copies for each chromosome in an embryonic sample. Combining the detection of chromosome copy number (CN) and proportion of maternal copies (%Mat) through APCAD allows to distinguish all types of chromosomal abnormalites independent of haplotyping.

In contrast to current methods, APCAD can detect the full spectrum of chromosome abnormalities, including haploidy, polyploidy and copy number neutral anomalies (UPD). APCAD guarantees a more accurate aneuploidy detection than simple CN-based methods. Moreover, using DNA samples of the prospective couple only - no family member samples are required - APCAD differentiates mosaic from complete, maternal from paternal and mitotic from meiotic CN abnormalities. In addition, the analysis can confirm that the gametes from the prospective parents were used. 

Authors

Pieter Verdyck

Organisations

VUB-US BRUSSEL

Presenting author

Pieter Verdyck, Molecular geneticist, VUB - UZ BRUSSEL
Pieter.Verdyck@uzbrussel.be
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