Multiplexing High-Throughput Flow Cytometry

During screening for NANOBODY® molecules, large amounts of clones are tested for the desired binding and functional properties. As this is a labor-intensive step, the High-Throughput Profiling Unit at Ablynx has been expanding its multiplexing capacity in order to maximize the throughput and efficiency of the screening campaigns. A multiplex assay setup that allows for assessment of the binding of NANOBODY samples towards recombinant protein-coupled beads and target-expressing cells in the same well, was established. By implementing this workflow, our team is able to manage the ever-increasing demand in screening during early-stage projects and simultaneously assess cross-reactivity, target specificity and other biologically-relevant properties (e.g. ligand competition).

Authors

Pieter Kennis, Kristof Moonens, Evelien Mathieu, Melanie Sanders, Jorn Audiens, Miguel Conde

Organisations

Ablynx, a Sanofi company

Presenting author

Pieter Kennis, Senior Scientist, Ablynx, a Sanofi company
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