Nanopore-based targeted sequencing for (epi)genetic typing applications

OHMX.bio is an enthusiastic young company, which helps customers solve their biological questions through cutting-edge technologies over different OMICS fields.

One of the flagships in its technology portfolio is nanopore-based long read sequencing (third generation sequencing). This groundbreaking sequencing technology has several unprecedented advantages for the analysis of DNA and RNA. First of all, its ultra-long reads allow better genomic (re-)assembly and novel splice isoform detection. Furthermore, the protocols are amplification-free and readouts allow direct epigenetic detection. With this revolutionary platform available at OHMX.bio, we focused on the implementation and optimization of PCR-free targeted sequencing approaches. Two targeted methods can now be routinely performed at OHMX.bio. The first one uses CRISPR-Cas9 technology in combination with nanopore sequencing in order to enrich read-out on a specific gene (set). The other technique applies adaptive nanopore control during sequencing to select in real time for different larger regions of interest in the genome. We applied both approaches of targeted sequencing for several use cases over different life science application fields. In a clinical setting, targeted third generation sequencing was employed to improve (rare) disease typing. This typing can be performed at the genetic level as well as on the epigenetic level in the same sequencing experiment due to the advantageous direct epigenetic readout which is offered by the nanopore sequencing. Furthermore, targeted sequencing provides also advantages for microbial use cases, where the targeted sequencing can help in microbial typing. On top of that, the adaptive pore control technique can also help in enriching low-abundant species in metagenomic samples.

Authors

Steven Verbruggen (1)

Organisations

OHMX.bio, Ghent (1)

Presenting author

Steven Verbruggen, Senior Bioinformatician, OHMX.bio
Steven.Verbruggen@ohmx.bio
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